{"product_id":"bronchogen-research-peptide","title":"Bronchogen","description":"\u003cp\u003e\u003cstrong\u003eCertificate of Analysis (COA): See Images\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBronchogen (Ala-Glu-Asp-Leu)\u003c\/strong\u003e is a synthetic tetrapeptide best known in the research literature for its interaction with DNA. First synthesised from a sequence associated with bronchial mucosa, this ultra-short Khavinson bioregulator has been studied for its apparent ability to enter the cell nucleus and influence the expression of genes tied to respiratory epithelial identity. While not approved for human or veterinary use, Bronchogen is widely used as a research peptide in laboratory and academic studies of peptide-DNA interaction and tissue-specific gene regulation.\u003c\/p\u003e\n\u003ch3\u003eResearch Applications\u003c\/h3\u003e\n\u003cp\u003eLaboratory investigation of Bronchogen spans molecular, cellular, and gene-expression studies, including:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003ePeptide-DNA Binding:\u003c\/strong\u003e Molecular studies report that the AEDL sequence interacts with DNA at the guanine N7 site in the major groove, apparently without distorting the double-helix structure, and influences DNA thermostability.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLung-Associated Gene Activation:\u003c\/strong\u003e In bronchial epithelial cell cultures, Bronchogen has been reported to increase expression of transcription factors and markers including NKX2-1, FOXA1, FOXA2 and SCGB1A1, with reported effects more pronounced in older (later-passage) cultures.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEpithelial Cell Kinetics:\u003c\/strong\u003e Studied in cell-culture models for its influence on the proliferation and differentiation of bronchial epithelial cells.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eBecause its activity centres on direct gene regulation rather than receptor signalling, Bronchogen is often used as a model compound for studying how ultra-short peptides reach and act on nuclear DNA. Researchers working with it frequently study it in parallel with other Khavinson bioregulators such as \u003ca href=\"https:\/\/tidelabs.co.uk\/products\/cortagen-research-peptide\"\u003eCortagen\u003c\/a\u003e and \u003ca href=\"https:\/\/tidelabs.co.uk\/products\/pancragen-research-peptide\"\u003ePancragen\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch3\u003eTechnical Information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eChemical Name:\u003c\/strong\u003e L-Alanyl-L-α-glutamyl-L-α-aspartyl-L-leucine\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSynonyms:\u003c\/strong\u003e AEDL, H-Ala-Glu-Asp-Leu-OH, Bronchogen\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular Formula:\u003c\/strong\u003e C₁₈H₃₀N₄O₉\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular Weight:\u003c\/strong\u003e 446.45 g\/mol\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSequence (Amino Acid):\u003c\/strong\u003e Ala-Glu-Asp-Leu (AEDL)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCAS Number:\u003c\/strong\u003e Not assigned\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePeptide Classification:\u003c\/strong\u003e Synthetic Khavinson organ-specific short peptide bioregulator (tetrapeptide)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eStorage Guidelines\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eLyophilized peptide is stable at room temperature for several weeks.\u003c\/li\u003e\n\u003cli\u003eFor long-term storage, keep at −20°C or below.\u003c\/li\u003e\n\u003cli\u003e\n\u003cmeta charset=\"UTF-8\"\u003e\n\u003cp class=\"p1\"\u003eOnce reconstituted, store at 2–8 °C. Effective use depends on the solution used.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eSolubility\u003c\/h3\u003e\n\u003cp\u003eBronchogen dissolves readily in \u003ca href=\"https:\/\/tidelabs.co.uk\/products\/bacteriostatic-water-10ml\"\u003ebacteriostatic water\u003c\/a\u003e, with reconstitution in standard aqueous buffers also reported in the literature.\u003c\/p\u003e\n\u003ch3\u003eReferences\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eMonaselidze, J., Khavinson, V., Gorgoshidze, M., et al. (2011). \"Effect of the Peptide Bronchogen (Ala-Asp-Glu-Leu) on DNA Thermostability.\" \u003cem\u003eBulletin of Experimental Biology and Medicine\u003c\/em\u003e, 150(3), 375–377. \u003ca rel=\"noopener\" href=\"https:\/\/doi.org\/10.1007\/s10517-011-1146-x\" target=\"_blank\"\u003ehttps:\/\/doi.org\/10.1007\/s10517-011-1146-x\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eFedoreyeva, L. I., Kireev, I. I., Khavinson, V. K., \u0026amp; Vanyushin, B. F. (2011). \"Penetration of short fluorescence-labeled peptides into the nucleus in HeLa cells and in vitro specific interaction of the peptides with deoxyribooligonucleotides and DNA.\" \u003cem\u003eBiochemistry (Moscow)\u003c\/em\u003e, 76(11), 1210–1219. \u003ca rel=\"noopener\" href=\"https:\/\/doi.org\/10.1134\/S0006297911110022\" target=\"_blank\"\u003ehttps:\/\/doi.org\/10.1134\/S0006297911110022\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eKhavinson, V. K., Popovich, I. G., Linkova, N. S., et al. (2021). \"Peptide Regulation of Gene Expression: A Systematic Review.\" \u003cem\u003eMolecules\u003c\/em\u003e, 26(22), 7053. \u003ca rel=\"noopener\" href=\"https:\/\/doi.org\/10.3390\/molecules26227053\" target=\"_blank\"\u003ehttps:\/\/doi.org\/10.3390\/molecules26227053\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Tide Labs","offers":[{"title":"10mg","offer_id":53709612777815,"sku":null,"price":19.5,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0941\/3142\/1527\/files\/Bronchogen10mgvial.webp?v=1780589507","url":"https:\/\/tidelabs.co.uk\/products\/bronchogen-research-peptide","provider":"Tide Labs ","version":"1.0","type":"link"}