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The Metabolic Signaling Research Set is a dual-reagent kit featuring high-concentration NAD⁺ (1000mg) and 5-Amino-1MQ (10mg). This pairing is engineered for laboratories investigating the NAD+ salvage pathway and mitochondrial bioenergetics.

The synergy of this kit allows for the study of intracellular coenzyme levels from two distinct vectors. While exogenous NAD⁺ provides the direct substrate for sirtuin activation and ATP production, 5-Amino-1MQ acts as a membrane-permeable inhibitor of the enzyme Nicotinamide N-methyltransferase (NNMT). Research suggests that by blocking NNMT, 5-Amino-1MQ prevents the degradation of nicotinamide, theoretically preserving the endogenous pool of NAD⁺.

Areas of Research Interest

• Mitochondrial Bioenergetics: Evaluation of ATP production efficiency and electron transport chain stability.
• NAD+ Flux Dynamics: Investigating the interplay between direct coenzyme supplementation and enzymatic inhibition of the salvage pathway.
• Adipocyte Metabolism: 5-Amino-1MQ is widely studied in models of white adipose tissue to observe shifts in cellular energy expenditure.
• Cellular Rejuvenation: Exploring the role of sirtuin signaling and DNA repair mechanisms in aged tissue models.

Technical Specifications

Component 1: NAD⁺ (1000mg)

• Format: 1000mg Lyophilized Cake
• Chemical Name: Nicotinamide Adenine Dinucleotide (oxidized form)
• Molecular Formula: C₂₁H₂₇N₇O₁₄P₂
• Molecular Weight: 663.43 g/mol
• CAS Number: 53-84-9
• PubChem CID: 5893

Component 2: 5-Amino-1MQ (10mg)

• Format: 10mg Lyophilized Powder
• Chemical Name: 5-amino-1-methylquinolinium
• Classification: Selective NNMT Inhibitor / Small Molecule
• Molecular Weight: 159.21 g/mol
• CAS Number: 42464-96-0

Storage & Stability Protocols

To maintain sequence integrity and prevent denaturation, both compounds are supplied in Type 1 Hydrolytic Glass. Tide Labs utilizes a strict cold-chain infrastructure for all metabolic reagents.

• Long-Term Storage (Lyophilized): Store at −20°C.
• Reconstitution: Reagents are soluble in Bacteriostatic Water (10ml) or sterile saline.
• Post-Reconstitution: Store at 2–8°C. Rapid use is recommended to ensure data accuracy.

Technical References

• Neelakantan, H., et al. (2019). “Selective and membrane-permeable small molecule inhibitors of nicotinamide N-methyltransferase reverse diet-induced obesity in mice.” Nature Medicine. https://doi.org/10.1038/s41591-019-0604-9
• Verdin, E. (2015). “NAD⁺ in aging, metabolism, and neurodegeneration.” Science. https://doi.org/10.1126/science.aac4854
• Campagna, R., et al. (2021). “Nicotinamide N-methyltransferase (NNMT) in health and disease.” Cellular and Molecular Life Sciences. https://doi.org/10.1007/s00018-021-03909-6

For further technical definitions, visit our Technical Index. Batch-specific testing data is available via our Knowledge Hub.